Haptoglobin Electrochemical Diagnostic Method for Subclinical Mastitis Detection in Bovine Milk

This work proposes an outstanding screening magneto-bioassay for the early identification of bovine subclinical mastitis. Haptoglobin (Hp) was used as a promising biomarker diagnosis To this end, novel chitosan-modified magnetic particles, coated with haemoglobin-modified polyaniline (MNPs@Chi/PANI-Hb), have been integrated in sensitive electrochemical Hp bioassay. Haemoglobin bioreceptor due to its high affinity against Hp. The appropriateness their synthesis and modifications were demonstrated by XRD, FT-IR spectroscopy, thermogravimetry N2 adsorption–desorption analyses. After optimization experimental parameters main analytical features obtained showing excellent performance. biosensor milk matrix presented dynamic range 0.001 0.32 μg mL−1 detection limit 0.031 mL−1, value much lower than normal values milk, making it highly suitable such determinations. Finally, real samples, from local dairy farmers, analysed bioassay compared commercial ELISA kit detection. Milk samples correctly classified “acceptable” or “unacceptable” considering concentration proposed bioassay, confirming predictive capacity mastitis diagnosis.